The protein Argonaute, which helps cells control protein production in a process called RNA interference. Credit: CSHL, in conjunction with Scripps Research
Scientists are working to better understand the RNA interference process, which could help improve treatments, such as cancer therapies. Recently, researchers discovered how the Argonaute protein is able to keep protein production on track by leveraging limited resources. This basic research breakthrough could help lead to advanced new therapies in the future.
Cells produce proteins like little factories. But if they make too much at the wrong times it can lead to diseases like cancer, so they control production with a process called RNA interference (RNAi). As of July 2021, several drugs already take advantage of RNAi to treat painful kidney and liver diseases—with another seven in clinical trials. There is a lot of potential for RNAi therapeutics, and Cold Spring Harbor Laboratory (CSHL) researchers are working hard to paint a complete picture of the process, to improve therapies today and make better ones tomorrow.
RNA interference (RNAi) is a biological process in which RNA molecules participate in the sequence-specific suppression of gene expression by double-stranded RNA, through translational or transcriptional repression.
CSHL Professor & Howard Hughes Medical Institute (HHMI) Investigator Leemor Joshua-Tor and recent CSHL School of Biological Sciences graduate Brianna Bibel are filling in some of the blanks. They recently discovered how RNAi’s workhorse protein Argonaute (Ago) leverages limited resources to keep protein production on track.
It’s important to understand exactly how RNAi works because it’s such a basic and heavily used process, Joshua-Tor said. It also offers a kind of safety net for therapeutics because it doesn’t make permanent changes to cells and can be reversed. Joshua-Tor says:
“For therapeutics, you’d kinda maybe not wanna mess around with the genome so much. In all these kinds of things, you wanna know exactly what’s happening, and if something isn’t working, then you know what to do and where to look. The more information you have, the better it is—you get a complete picture of what’s happening.”
As essential components of the RNA-induced silencing complex (RISC), Argonaute protein family plays a central role in RNA silencing processes.
Ago helps cut off protein production by finding, binding, and destroying molecules called mRNA—which tell cells to make proteins. But the amount of Ago in the body pales in comparison to the amount of mRNA it must target. After destroying one, the protein is still capable of finding another but it can’t move on without help. Bibel discovered how cells use a process called phosphorylation to break Ago’s grip on an mRNA target, allowing it to commute to the next. Bibel explains:
“Our theory is that having phosphorylation promote release is a way that you could free up Argonaute because when the target gets released, the guide’s still there and it’s super duper stable. So our thinking is that by phosphorylating it, you’re going to free it to go repress other targets—because it’s still totally capable of doing that work.”
Bibel hopes her discovery will come in handy as research into RNAi continues. “A lot of great advances in science come from just doing basic research,” she said. “And this is one of those basic research questions, trying to figure out how this is working.”
Reference: “Target binding triggers hierarchical phosphorylation of human Argonaute-2 to promote target release” by Brianna Bibel, Elad Elkayam, Steve Silletti, Elizabeth A Komives and Leemor Joshua-To, 31 May 2022, eLife.
DOI: 10.7554/eLife.76908
Funding: National Science Foundation, Howard Hughes Medical Institute